Mrs Dorothy Kisaka studied at Gayaza High school from 1977 to 1983 , residing in Hutchinson House. Dorothy graduated from Makerere University with a Bachelor of Laws degree in 1987. She followed that by obtaining a Diploma in Legal Practice from the Law Development Centre. She was then admitted to the Uganda Bar. Her second degree is a Master of Arts in Organisational Leadership and Management, obtained from Uganda Christian University, in Mukono, Uganda. Her third degree is also a Master of Arts degree, awarded by York St John University, in the United Kingdom.

From 1999 until 2014, she was an Associate Attorney at Kiyimba Kisaka & Company Advocates in Kampala. From October 2010 until December 2014, Dorothy Kisaka also served as a commissioner at the Electoral Commission of Uganda. She was then appointed as a presidential advisor and subsequently a Senior Presidential Advisor.

In April 2020, Yoweri Museveni, the President of Uganda, appointed Dorothy Kisaka as secretary of the COVID-19 Response Fund. In July 2020 Dorothy Kisaka was sworn in as the New Executive Director of Kampala Capital City Authority for a three year term. Dr Kisaka pledged to lead from the front and lead by example.
Dorothy also currently serves as Chairperson of The Development Associates International and represents Africa on the Board of Haggai International